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Rapid method for the analysis of urinary pyrrole-2-carboxylic acid using reversed-phase high-performance liquid chromatography

โœ Scribed by Joseph H. Dreisbach; Andrea Veca


Book ID
108449580
Publisher
Elsevier Science
Year
1986
Weight
232 KB
Volume
382
Category
Article
ISSN
0378-4347

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A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reversephase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stabilit