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Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography

✍ Scribed by E. Bousquet; V. Guarcello; M.C. Morale; V. Rizza

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 385 KB
Volume: 131
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90144-6

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✦ Synopsis

A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reversephase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability. and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity.

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