Rapid melting apparatus for studying thermal denaturation of polynucleotides

✍ Scribed by B.F. Rordorf; D.R. Kearns

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 476 KB
• Volume: 71
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90024-5

✦ Synopsis

A spectrophotometer attachment is described which allows complete thermal denaturation of heating/cooling cycles (0 to 90 to 0°C) of polynucleotides to be obtained in less than 5 min. Solutions with a wide range of optical densities (0.15 to 500) can be studied and several examples are given to demonstrate the performance and application of the device.

Methods

A model HG 201B heat gun (500 W) by Master Appliance Corp., Racine, Wis., was used as the hot air source. Stainless steel coated subminature copper-constantan thermocouple assemblies (Model SCPSS-020G-6, of 0.020 in. diameter by Omega Engineering, Inc., Stamford, Conn.) were used for the temperature measurement directly in the solu-172 Copyright