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Rapid isolation of highly productive recombinant Chinese hamster ovary cell lines

โœ Scribed by Miloux, Brigitte; Lupker, Jan H.

Book ID
122001465
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
442 KB
Volume
149
Category
Article
ISSN
0378-1119

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Isolation of Chinese hamster ovary cell
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โœ Yucheng Zeng; Mark A. Lehrman ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 714 KB

Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produce