Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans

✍ Scribed by Yucheng Zeng; Mark A. Lehrman

• Book ID: 102985178
• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 714 KB
• Volume: 193
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(91)90020-t

✦ Synopsis

Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.