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Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans

โœ Scribed by Yucheng Zeng; Mark A. Lehrman


Book ID
102985178
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
714 KB
Volume
193
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.


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