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Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions

✍ Scribed by C. H. C. M. Buys; J. Osinga; W. L. Gouw; G. J. P. A. Anders

Publisher
Springer
Year
1978
Tongue
English
Weight
634 KB
Volume
44
Category
Article
ISSN
0340-6717

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✦ Synopsis

The use of a combination of transmitted light and epiluminescence after silver and fluorescent staining of chromosome preparations makes it possible to achieve simultaneous visualization of silver-stained NORs and flourescent chromosomes. This technique permits exact localization of silver precipitates on normal and BrdU-substituted chromosomes. After previous silver impregnation, fluorescent staining by actinomycin-daunomycin-DAPI was used to induce a banding pattern that enables identification of specific chromosomes while observing silver-stained NORs at the same time. Application of this method to a Down's syndrome patient revealed a 21/21 Robertsonian translocation with NOR'S eliminated.

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