✦ LIBER ✦

Rapid identification and isolation of zygotes and the kinetics of mating inSaccharomyces cerevisiae

✍ Scribed by David E. Block; Friedrich Srienc

Publisher: Springer-Verlag
Year: 1991
Tongue: English
Weight: 448 KB
Volume: 5
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00159978

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✦ Synopsis

Parental cells of opposite mating type were stained with different fluorescent dyes, permitting rapid identification of zygotes from natural matings based on dual-color flow cytometry. This procedure was then used to study the kinetics of mating in yeast and the physical and biological parameters that affect these kinetics, such as cell concentration, parental ratios, and parental strain growth rate.

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