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Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid

✍ Scribed by Aurelius, E.; Johansson, B.; Staland, A.; Forsgren, M.; Aurelius, E.; Skoldenberg, B.

Book ID: 121286016
Publisher: The Lancet
Year: 1991
Tongue: English
Weight: 554 KB
Volume: 337
Category: Article
ISSN: 0140-6736
DOI: 10.1016/0140-6736(91)92155-U

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## Abstract Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumber cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR‐SB results