✦ LIBER ✦

Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

✍ Scribed by Dr. Paul E. Klapper; Graham M. Cleator; Carol Dennett; Alfor G. Lewis

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 454 KB
Volume: 32
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890320413

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumber cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR‐SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant “non‐invasive” method for the diagnosis of HSE.

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