Rapid determination of disaccharides from chondroitin and dermatan sulphates by high-performance liquid chromatography
✍ Scribed by J. Macek; J. Krajíčková; M. Adam
- Publisher
- Elsevier Science
- Year
- 1987
- Weight
- 312 KB
- Volume
- 414
- Category
- Article
- ISSN
- 0378-4347
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An improved high-performance liquid chromatography for unsaturated disaccharides prepared from chondroitin sulfate and dermatan sulfate isomers was developed using an ion-exchange resin made from a sulfonized styrene-divinylbenzene copolymer. By this newly devised method, it was found that the reten
A high-performance liquid chromatography method for analyzing disaccharides derived from chondroitin sulfate glycosaminoglycans has been developed which employs a Whatman Partisil-10 PAC amino-cyano column and an acetonitrile/methanol/ammonium acetate solvent to resolve disulfated, monosulfated, and
Various under-sulfated, monosulfated, and over-sulfated chondroitin sulfate and dermatan sulfate isomers were analyzed in terms of disaccharide units before or after desulfation with chondrosulfatases in addition to digestion with chondroitinases. The unsaturated disaccharides were separable by a hi