Enzymatic analysis with chondrosulfatases of constituent disaccharides of sulfated chondroitin sulfate and dermatan sulfate isomers by high-performance liquid chromatography

✍ Scribed by Katsumi Murata; Y. Yokoyama

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 689 KB
• Volume: 149
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90503-2

✦ Synopsis

Various under-sulfated, monosulfated, and over-sulfated chondroitin sulfate and dermatan sulfate isomers were analyzed in terms of disaccharide units before or after desulfation with chondrosulfatases in addition to digestion with chondroitinases. The unsaturated disaccharides were separable by a high-performance liquid chromatography (HPLC) method using a resin made from a sulfonized styrene-divinylbenzene copolymer. The retention times of the parent sulfated unsaturated disaccharides and newly generated unsaturated mono-or nonsulfated disaccharides were reproducible. On desulfation of the parent sulfated unsaturated disaccharides with chondrosulfatases, almost all ADi-S showed the same retention times as those of standard ADi-S from known components. Following digestion of ADi-diSa with chondro-4-sulfa&se as well as ADi-diSo or ADi-diSo with chondro-6-sulfatase, three ADi-monoS with the same retention time were detected with the HPLC method. These newly generated ADi-mono$ showed that the structure is N-acetyl-D-galactosamine, uranic acid 2-sulfate. o 1985 Academic Press, Inc. KEY WORDS: high-performance liquid chromatography of sulfated chondroitin and dermatan sulfate isomers; chondrosulfatases; constitutional disaccharides of variously sulfated chondroitin and dermatan sulfate isomers.

Glycosaminoglycans (GAG)' such as chondroitin sulfate and dermatan sulfate isomers in intercellular matrices show a certain het-