โ Scribed by Nicola Volpi
No coin nor oath required. For personal study only.
A system capable of resolving all the known unsaturated nonsulfated, mono-and disulfated disaccharides derived from chondroitin sulfate samples, dermatan sulfate, and hyaluronic acid after their derivatization with dansylhydrazine and separation by HPLC and fluorimetric detection is reported. This method was found superior to others in that unsaturated disaccharides can be separated with good resolution in about 50 min in an isocratic solvent with a sensitivity greater than about 50 pmol (approx 20 -30 ng) and linearity from 50 to 500 pmol. The system was applied to the analysis of various chondroitin sulfate samples, including highly sulfated species and dermatan sulfate, and also to a defructosylated polysaccharide with a chondroitin backbone purified from Escherichia coli U1-41. Excellent agreement was obtained with traditional compositional analysis performed by anion-exchange HPLC separation and UV absorption at 230 nm.
๐ SIMILAR VOLUMES
## Abstract CE conditions for monitoring the unsaturated disaccharides of hyaluronic acid (diโHA) and chondroitin sulfate (diโCS) using an alkaline tetraborate buffer, electrokinetic sample injection, and UV absorption detection at 232 nm are reported. Separations were performed in an uncoated fuse
Separation of unsaturated fatty acids and their hydroperoxides and hydroxides was carried out easily and quickly by high-performance liquid chromatography on the porous polymer gel (TSK-Gel LS-140) using n-hexaneiethanol as an eluent. This method was proved to be useful for the quantitative analysis