Rapid Cycle Real-Time PCR — Methods and Applications: Quantification

✍ Scribed by Onno Bakker, Daphne C. Timmer (auth.), Professor Dr. Carl Wittwer, Dr. Meinhard Hahn, Dr. Karen Kaul (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2004
• Tongue: English
• Leaves: 218
• Edition: 1
• Category: Library

✦ Synopsis

Rapid Cycle Real-Time PCR is a powerful technique for nucleic

acid quantification and analysis that takes less than 30 minutes

to complete. Fluorescence is automatically monitored

each cycle and the amount of template quantified by advanced

analytical methods, such as the second derivative maximum method.

Immediately following rapid cycle PCR, melting curve analysis is

performed to verify product purity with SYBR Green I and/or

genotype with fluorescently-labeled hybrdidization probes

(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often

cited as the most versatile, efficient method for nucleic acid

quantification in research and climical studies. Molecular analysis

has never been easier!

✦ Table of Contents

Front Matter....Pages I-VII
Front Matter....Pages 1-1
Housekeeping Genes: A Gold Standard?....Pages 3-10
The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia....Pages 11-20
Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach....Pages 21-29
Measuring Genome Sizes by Absolute Quantification....Pages 31-41
Front Matter....Pages 43-43
Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I....Pages 45-51
Quantification of δRec-ψJα Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation....Pages 53-59
Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR....Pages 61-72
Quantification of Ikaros Splice Variants by Real-Time PCR....Pages 73-82
Methods to Quantify Cytokine Gene Expression by Real-Time PCR....Pages 83-92
Profiling Breast Cancer Using Real-Time Quantitative PCR....Pages 95-106
HER-2/neu Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue....Pages 107-116
Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer....Pages 117-124
A Rapid Cycle Real-Time Quantitative β-globin PCR for Quantification of Human DNA in Feces....Pages 125-130
Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycler™....Pages 131-140
Real-Time Quantification of the AML Rearrangements, AML1-ETO and TEL-AML1 , in Acute Leukemia....Pages 141-155
Rapid Detection of Gene Duplications in Charcot-Marie-Tooth 1A Disease by SNP Genotyping Using Real-Time PCR....Pages 159-169
Cytochrome P450 2D6 Deletion Genotyping Using Derivative Curve Analysis on the LightCycler....Pages 171-178
Trisomy 21 Detected by SNP Allele Ratios....Pages 179-186
Quantitative Chimerism Analysis by Allele-Specific Real-Time PCR of a 10 bp Insertion/Deletion Polymorphism within the Promotor Region of Factor Vllc....Pages 187-195
Detection of SARS-Coronavirus in the LightCycler by 5′-Nuclease Real-Time RT-PCR....Pages 199-210
Front Matter....Pages 43-43
Normalized Quantitative Rapid-Cycle Real-Time PCR for the Assessment of CMV-DNA Copies....Pages 211-223

✦ Subjects

Biochemistry, general