Rapid Cycle Real-Time PCR — Methods and Applications: Microbiology and Food Analysis

✍ Scribed by Franklin Cockerill (auth.), Dr. Udo Reischl, Prof. Dr. Carl Wittwer, Prof. Dr. Franklin Cockerill (eds.)

Publisher: Springer-Verlag Berlin Heidelberg
Year: 2002
Tongue: English
Leaves: 252
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interested for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

✦ Table of Contents

Front Matter....Pages I-IX
Introduction for Microbiology Volume of Rapid Cycle Real-Time PCR....Pages 1-2
Applications and Challenges of Real-Time PCR for the Clinical Microbiology Laboratory....Pages 3-27
Front Matter....Pages 29-30
Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimens by LightCycler PCR....Pages 31-43
Rapid Detection and Simultaneous Differentiation of Legionella spp. and L. pneumophila in Potable Water Samples and Respiratory Specimens by LightCycler PCR....Pages 45-57
Homogeneous Assays for the Rapid PCR detection of Burkholderia pseudomallei 16S rRNA gene on a Real-Time Fluorometric Capillary Thermocycler....Pages 59-69
Rapid Detection of Toxigenic Corynebacterium diphtheriae by LightCycler PCR....Pages 71-81
Rapid Detection of Resistance Associated Mutations in Mycobacterium tuberculosis by LightCycler PCR....Pages 83-91
Duplex LightCycler PCR Assay for the Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation....Pages 93-105
Rapid Detection of Group B Streptococci Using the LightCycler Instrument....Pages 107-114
Rapid Detection and Quantification of Chlamydia trachomatis in Clinical Specimens by LightCycler PCR....Pages 115-132
Quantification of Toxoplasma gondii in Amniotic Fluid by Rapid Cycle Real-Time PCR....Pages 133-138
Genospecies-Specific Melting Temperature of the recA PCR Product for the Detection of Borrelia burgdorferi sensu lato and Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto....Pages 139-147
Rapid and Specific Detection of Coxiella burnetii by LightCycler PCR....Pages 149-154
Molecular Identification of Campylobacter coli and Campylobacter jejuni Using Automated DNA Extraction and Real-Time PCR with Species-Specific TaqMan Probes....Pages 155-159
Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR....Pages 161-169
Quantification of Thermostable Direct Hemolysin-Producing Vibrio Parahaemolyticus from Foods Assumed to Cause Food Poisoning Using the LightCycler Instrument....Pages 171-176
Front Matter....Pages 177-177
Quantification and Speciation of Fungal DNA in Clinical Specimens Using the LightCycler Instrument....Pages 179-186
Front Matter....Pages 187-187
Development, Implementation, and Optimization of LightCycler PCR Assays for Detection of Herpes Simplex Virus and Varicella-Zoster Virus from Clinical Specimens....Pages 189-200
Qualitative Detection of Herpes Simplex Virus DNA in the Routine Diagnostic Laboratory....Pages 201-209
Use of Real-Time PCR to Monitor Human Herpesvirus 6 (HHV-6) Reactivation....Pages 211-216
Front Matter....Pages 187-187
Simultaneous Identification of Five HCV Genotypes in a Single Reaction....Pages 217-225
Rapid Detection of West Nile Virus....Pages 227-234
Use of Rapid Cycle Real-Time PCR for the Detection of Rabies Virus....Pages 235-240
Development of One-Step RT-PCR for the Detection of an RNA Virus, Dengue, on the Capillary Real-Time Thermocycler....Pages 241-248
Front Matter....Pages 249-249
Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products (RRS, Bt176, Bt11, Mon810, T25, Lectin, Invertase)....Pages 251-258

✦ Subjects

Molecular Medicine

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