Rapid Microbiological Methods for Foods, Beverages and Pharmaceuticals

✍ Scribed by C. J. Stannard, S. B. Petitt, F. A. Skinner

• Publisher: Wiley-Blackwell
• Year: 1991
• Tongue: English
• Leaves: 320
• Edition: 1
• Category: Library

✦ Synopsis

This book considers the rapid microbiological techniques that are now increasingly used in industry as alternatives to more conventional methods. Although many of the pioneering studies in this field have taken place in clinical laboratories, the materials listed and organisms sought for foods, beverages and pharmaceuticals are much more varied. In this volume, leading experts from research and industry review the wide variety of approaches that are needed in an industrial setting. The methods described include electrometric techniques, ATP assay, and immunological methods for a wide range of organisms from salmonellas to viruses, each chapter drawing on the authors direct experience in industry to give a highly practical guide. The book should prove invaluable to those in the food, beverage and pharmaceutical industries, or in research and training, who require an up-to-date survey of the use of rapid microbiological methods.

✦ Table of Contents

Rapid Microbiological Methods for Foods, Beverages and Pharmaceuticals......Page 1
Contents......Page 7
Contributors......Page 12
Preface......Page 15
The Use of ATP Bioluminescence for the Analysis of Beer in Polyethylene Terephthalate (PET) Bottles and Associated Plant......Page 17
Materials and Methods......Page 18
Results......Page 22
Discussion......Page 25
References......Page 26
Rapid Assessment of the Bacterial Content of Milk by Bioluminescent Techniques......Page 29
Materials and Methods......Page 30
Results......Page 33
Discussion......Page 42
References......Page 45
Apparatus, Reagents and Methodology......Page 49
Selective Pre-incubation for the Detection of Low Levels of Spoilage Bacteria by the DEFT......Page 52
Osmotolerant Yeasts in Confectionery Products......Page 54
Selective Enumeration of Bacteria by the DEFT......Page 55
Use of DEFT for Irradiated Foods......Page 58
References......Page 60
DEFT Methodology for Meat and Poultry......Page 63
Comparison of DEFT and Plate Counts......Page 68
Applicability of DEFT to Meat and Poultry......Page 71
References......Page 72
Urine Examination by the DEFT......Page 75
Analysis of Intravenous Fluids by the DEFT......Page 80
Conclusion......Page 85
References......Page 86
What is Image Analysis?......Page 89
Image Analysis at Brockham Park......Page 90
Application to Bioassay......Page 91
Application to MIC Determination......Page 94
References......Page 101
Optimization of Automated Electrometric Methods......Page 103
Curve Quality......Page 105
Comments on some Conventional Assays......Page 106
Calibration Curves......Page 107
Practical Experiments on Fish......Page 109
Inorganic Constituents......Page 112
References......Page 114
Conductance Techniques for the Detection of Contaminants in Beer......Page 117
General Procedures......Page 118
Screening of Non-Selective Media......Page 119
Detection Routine Evaluation......Page 120
Development of Selective Media......Page 121
Field Trial......Page 125
Discussion......Page 131
References......Page 133
Electrical Methods for Water Quality Testing......Page 135
Use of the Bactometer M123 for Environmental and Recreational Water Samples......Page 136
Use of the Bactometer M123 for Potable Water Samples......Page 139
Use of the Malthus Microbial Growth Analyser for Potable Water Analysis......Page 141
References......Page 146
A Conductance Screen for Enterobacteriaceae in Foods......Page 147
Rapid Tests for the Enterobacteriaceae......Page 148
Materials and Methods......Page 149
Results......Page 153
Discussion......Page 155
References......Page 156
Electrical Screening of Powdered Dairy Products......Page 159
Materials and Methods......Page 160
Results......Page 163
Discussion......Page 167
References......Page 169
An Inter-Laboratory Evaluation of an Electrometric Method for Detection of Salmonellas in Milk Powders......Page 171
Participating Laboratories......Page 172
Materials and Methods......Page 173
Comparison of the British Standard Method with the Easter-Gibson Method......Page 175
Results and Discussion......Page 176
References......Page 180
Rapid Salmonella Detection by a Combination of Conductance and Immunological Techniques......Page 181
Materials and Methods......Page 182
Results......Page 188
Discussion......Page 195
References......Page 198
Automated Conductimetric Detection of Salmonellas in Confectionery Products......Page 201
Description and Principles......Page 202
Method Evaluation......Page 211
Conclusion......Page 215
References......Page 216
Preparation of Skimmed Milk Powder Contaminated with Lancefield Group D Cocci......Page 219
Use of Conventional Selective Media in Electrometric Instruments......Page 221
Medium Development for Electrometric Detection......Page 222
Effect of Medium Composition on Curve Quality......Page 223
Calibration of Malthus and Bactometer Instruments......Page 225
References......Page 227
BIOCHECK - a Mediated Amperometric Microbial Activity Monitor......Page 229
Requirements for Amperometric Biomass Sensing......Page 230
Evaluation of Suitable Mediators......Page 233
Assessment of Electrodes......Page 235
Prototype Development......Page 236
Considerations for the Examination of Real Samples......Page 240
References......Page 241
Detection of Electron Transfer for the Assessment of Bacterial Contamination......Page 243
Materials and Methods......Page 245
Results and Discussion......Page 249
References......Page 255
Computer-Assisted Identification of Moulds......Page 257
The Evolution of Mould Identification......Page 258
Methods......Page 259
References......Page 264
Bio-Enzabead Screen Kit......Page 265
TECRA Salmonella Visual Immunoassay......Page 269
Kirkegaard and Perry Salmonella ELISA......Page 272
Salmonella 1-2 Test......Page 275
Discussion......Page 278
References......Page 279
Immunoassay Kits for the Detection of Toxins Associated with Foodborne Illness......Page 281
Materials and Methods......Page 282
Results and Discussion......Page 283
References......Page 296
Rapid Detection of Viruses in Water and the Water Environment......Page 299
Materials and Methods......Page 300
Discussion......Page 305
References......Page 306
Index......Page 309