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Quantitative Real-Time PCR: Methods and Protocols

✍ Scribed by Roberto Biassoni, Alessandro Raso

Publisher
Springer New York;Humana
Year
2020
Tongue
English
Leaves
241
Series
Methods in Molecular Biology 2065
Edition
2nd ed. 2020
Category
Library
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✦ Synopsis

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.

✦ Table of Contents

Front Matter ....Pages i-xiii
A Quarter Century of PCR-Applied Techniques and Their Still-Increasing Fields of Use (Alessandro Raso, Roberto Biassoni)....Pages 1-4
Parameters for Successful PCR Primer Design (Stephen A. Bustin, Reinhold Mueller, Tania Nolan)....Pages 5-22
MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing (Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, Michael W. Pfaffl)....Pages 23-38
Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A) Tailing and 5′ Adaptor Ligation (Valentin Vautrot, Isabelle Behm-Ansmant)....Pages 39-54
A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R (Priscilla Biswas, Eddi Di Marco, Mauro S. Malnati)....Pages 55-64
Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) (Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, Alice Gomes Fernandes-Monteiro)....Pages 65-77
Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) (Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, Belén Rodelas)....Pages 79-94
Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine (Nicola Luchi, Alberto Santini, Francesca Salvianti, Pamela Pinzani)....Pages 95-104
A General Protocol for Accurate Gene Expression Analysis in Plants (Ellen De Keyser, Laurence Desmet, Magali Losschaert, Jan De Riek)....Pages 105-118
Gene Expression Analysis in Bacteria by RT-qPCR (Danilo J. P. G. Rocha, Thiago L. P. Castro, Eric R. G. R. Aguiar, Luis G. C. Pacheco)....Pages 119-137
Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR (Francesca Salvianti, Filomena Costanza, Gemma Sonnati, Pamela Pinzani)....Pages 139-151
Molecular Monitoring of Chronic Myeloid Leukemia (Katherine Dominy, Katya Mokretar, Alistair G. Reid, Jamshid S. Khorashad)....Pages 153-173
Normalization in Human Glioma Tissue (Ana Paula Santin Bertoni, Isabele Cristiana Iser, Rafael Paschoal de Campos, Márcia Rosangela Wink)....Pages 175-190
qPCR Applications for the Determination of the Biological Age (Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello et al.)....Pages 191-197
QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis (Chiara Broccanello, Letizia Gerace, Piergiorgio Stevanato)....Pages 199-208
Digital PCR and the QuantStudio™ 3D Digital PCR System (Marion Laig, Christie Fekete, Nivedita Majumdar)....Pages 209-231
Back Matter ....Pages 233-235

✦ Subjects

Life Sciences; Genetics and Genomics; Human Genetics

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