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Quantitative PCR Protocols

โœ Scribed by Bernd Kochanowski, Udo Reischl

Publisher
Humana Press
Year
1999
Tongue
English
Leaves
296
Series
Methods in Molecular Medicine
Edition
1
Category
Library
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โœฆ Synopsis

This is a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. With its step-by-step instructions, Quantitative PCR Protocols allows researchers to address biological and diagnostic questions that are difficult or impossible to answer using any other experimental approach.

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