𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Rapid chromosome detection by PRINS in human sperm

✍ Scribed by Pellestor, Franck ;Imbert, Isabelle ;Andréo, Brigitte

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
127 KB
Volume
107
Category
Article
ISSN
0148-7299

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Aneuploidy detection in human sperm nucl
Aneuploidy detection in human sperm nuclei using PRINS technique
✍ Girardet, Anne; Coignet, Lionel; Andréo, Brigitte; Lefort, Geneviève; Charlieu, 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 539 KB

Rapid and specific identification of chromosomes can be attained in situ using the PRimed IN Situ (PRINS) labelling technique. We have adapted this technique to mature human sperm in combination with a protocol for simultaneous decondensation and denaturation of sperm nuclei. This strategy allowed u

PRINS as a method for rapid chromosomal
PRINS as a method for rapid chromosomal labeling on human spermatozoa
✍ Dr. Franck Pellestor; Anne Girardet; Geneviève Lefort; Brigitte Andréo; Jean Pau 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 512 KB

## Abstract Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a __Taq__ DNA polymerase. The reaction was carried out on a programmable temperature cycler, an

Incidence of chromosome 18 disomy in hum
Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization
✍ Martina Guttenbach; Renate Schakowski; Michael Schmid 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 505 KB

Nonradioactive in situ hybridization with an ctsatellite DNA probe specific for chromosome 18 was performed on human interphase sperm nuclei to detect the frequency of sperm cells disomic for chromosome 18. A total of 16127 sperm heads from eight healthy donors, aged 23-57 years, was investigated, a

Rapid identification of marker chromosom
Rapid identification of marker chromosomes using primed in situ labeling (PRINS)
✍ Velagaleti, G. V. N.; Tharapel, S. A.; Martens, P. R.; Tharapel, A. T. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 21 KB 👁 2 views

Primed in situ labeling (PRINS) is a relatively new technology with wide-ranging applications in clinical cytogenetics. Using PRINS, we have identified the chromosomal origin of marker chromosomes in three patients. In the first patient with primary amenorrhea, we were able to confirm the marker chr