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Aneuploidy detection in human sperm nuclei using PRINS technique

✍ Scribed by Girardet, Anne; Coignet, Lionel; Andréo, Brigitte; Lefort, Geneviève; Charlieu, Jean Paul; Pellestor, Franck

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
539 KB
Volume
64
Category
Article
ISSN
0148-7299

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✦ Synopsis

Rapid and specific identification of chromosomes can be attained in situ using the PRimed IN Situ (PRINS) labelling technique. We have adapted this technique to mature human sperm in combination with a protocol for simultaneous decondensation and denaturation of sperm nuclei. This strategy allowed us to obtain double labelling of human spermatozoa in a <2-hr reaction. In the present study, we report the estimates of disomy for chromosomes 3, 7, 10, 11, and 17 on 64,642 spermatozoa from 2 normal males. The incidences of disomy ranged from 0.28434%. There were no significant interindividual or interchromosomal differences in disomy rates. @

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## Abstract __**Aim:**__  To study the aneuploidy rates of chromosomes 13, 18, 21, X and Y in Percoll gradient centrifuged sperm from infertile patients with male infertility factor treated by intracytoplasmic sperm injection (ICSI) compared with healthy fertile donors and infertile patients with