Detection of aneuploidy in human spermatozoa using fluorescencein situhybridization (FISH)
โ Scribed by He Hu; Norio Miharu; Tomoya Mizunoe; Yoshiharu Nakaoka; Etsuji Okamoto; Koso Ohama
- Publisher
- Nature Publishing Group
- Year
- 1996
- Tongue
- English
- Weight
- 558 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1435-232X
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The paper describes an attempt to estimate the frequency of aneuploid human spermatozoa with disomic Y chromosome and disomic chromosome 1 complements, using chromosome specific probes and in situ hybridisation. This approach was used as an alternative to the differential staining techniques that ha
Fluorescence in situ hybridisation (FISH) with a hufrequencies of centromere-negative micronuclei did man alphoid satellite'pancentromeric DNA probe was used to detect centromeres in micronuclei of human lymphocytes induced by y irradiation and by Vinblastine sulfate. In a cytokinesis-block micronuc