Detection of centromeres in vinblastine- and radiation-induced micronuclei of human lymphocytes using FISH with an α satellite pancentromeric DNA probe
✍ Scribed by R. Huber; K. Salassidis; U. Kulka; H. Braselmann; M. Bauchinger
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 404 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Fluorescence in situ hybridisation (FISH) with a hufrequencies of centromere-negative micronuclei did man alphoid satellite'pancentromeric DNA probe was used to detect centromeres in micronuclei of human lymphocytes induced by y irradiation and by Vinblastine sulfate. In a cytokinesis-block micronucleus assay a dose-dependent increase of micronuclei was detected for both agents. 72-89% of Vinblastine-induced micronuclei, but only 7-48% of radiation-induced micronuclei showed centromere positive fluorescence signals. Vinblastine treatment not'increase compared to control values, nor did frequencies of centromere-positive micronuclei in irradiated lymphocytes. Since FISH with an a satellite DNA probe allows the direct detection of centromeric DNA sequences the spindle damaging or clastogenic effectiveness of a compound can be easily and reliably examined in a cytokinesis-block micronucleus assay in human lymphocytes.