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PRINS as a method for rapid chromosomal labeling on human spermatozoa

✍ Scribed by Dr. Franck Pellestor; Anne Girardet; Geneviève Lefort; Brigitte Andréo; Jean Paul Charlieu

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
512 KB
Volume
40
Category
Article
ISSN
1040-452X

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✦ Synopsis

Abstract

Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a Taq DNA polymerase. The reaction was carried out on a programmable temperature cycler, and labeling was obtained in a 1‐hr reaction. The method was successfully tested with specific primers for chromosomes 13, 16, and 21. This suggests that PRINS may be a fast and reliable technique for detecting aneuploidies. © 1995 Wiley‐Liss Inc.

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