A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien
Rapid and simple method for the quantitative extraction of corn endosperm proteins
β Scribed by Jose Madrid Concon
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 554 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A simple protein determination method is described which gives a linear response from 0.05 to 3-4 mg/ml (or more) of protein and is suitable for dilute (<0.05 mglml) protein solutions if concentrated on paper by repeated spotting. Five-microliter solutions of a standard protein and the unknown are s
The method described here is essentially a modification of the method of Dilley and Crane (1) but has the advantages of greater speed and simplicity and gives higher recoveries. ## MATERIALS Benzene, petroleum ether (S&80), diethyl ether, absolute ethanol, and acetone were all analytical reagent