A simple and rapid method for the quantitative determination of α-tocopherol (vitamin E) in leaves

✍ Scribed by P.G. Roughan

• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 863 KB
• Volume: 19
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(67)90237-0

✦ Synopsis

The method described here is essentially a modification of the method of Dilley and Crane (1) but has the advantages of greater speed and simplicity and gives higher recoveries.

MATERIALS

Benzene, petroleum ether (S&80), diethyl ether, absolute ethanol, and acetone were all analytical reagent grade. Chloroform was redistilled commercial grade, and commercial grade 95% ethanol was refluxed over KOH and KMnO.+ (2 gm and 1 gm per liter, respectively) for 30 min before collecting the fraction distilling at 77-78°C. Sodium borohydride, ferric chloride (A.R.) and 2,2-dipyridyl (A.R.) were obtained from British Drug Houses, Ltd. Standard a-tocopherol was prepared by preparative thin-layer chromatography from nn-a-tocopherol (C-Grade, Calbiochem, Los Angeles).