## Abstract Investigation of hepatitis A virus (HAV) outbreaks often implies nucleotide sequence analysis. As an alternative method for the identification of related strains, single strand conformation polymorphism method (SSCP) was compared to sequence analysis. Twentyβthree strains from sporadic
Rapid and sensitive genotyping of hepatitis C virus by single-strand conformation polymorphism
β Scribed by Ricky R. Lareu; Nigel R. Swanson; Simon A. Fox
- Book ID
- 114140326
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 929 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0166-0934
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In patients infected with the hepatitis C virus (HCV), a heterogeneous population of viruses, so-called quasispecies exists in vivo. The hypervariable regions (HVR) within the second envelope gene (HCV-E2) show particularly highly intratypic variability and are considered to be the target of neutral
## Abstract This study describes a novel method of ABO genotyping using amplicons generated by PCRβSSCP and separated by CE. We describe four amplicons with sizes of 112, 121, 123 and 160βbp based on SSCP analysis. These amplicons are amplified from exons 6 and 7 and include polymorphic sites of cD