## Abstract This study describes a novel method of ABO genotyping using amplicons generated by PCRβSSCP and separated by CE. We describe four amplicons with sizes of 112, 121, 123 and 160βbp based on SSCP analysis. These amplicons are amplified from exons 6 and 7 and include polymorphic sites of cD
Genotyping Taenia tapeworms by single-strand conformation polymorphism of mitochondrial DNA
β Scribed by Robin B. Gasser; Xingquan Zhu; Wayne Woods
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 91 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
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Polymerase chain reaction (PCR)-coupled single-strand conformation polymorphism (SSCP) approaches utilizing nuclear DNA regions of the small subunit (SSU) of ribosomal RNA and heat shock protein 70 gene (HSP70) were established for genotyping Cryptosporidium parvum. The regions were amplified (indiv
## Abstract Using a singleβstrand conformation polymorphismβbased approach, we investigated nucleotide variation in part of the first internal transcribed spacer (pITSβ1) of nuclear ribosomal DNA within and among a large number of __Ascaris__ individuals from humans and pigs from six endemic region