An efficient and convenient procedure for the hydrolysis of bile acid methyl esters is described. This is achieved by the addition of aqueous lithium hydroxide in methanol/dioxane/tetrahydrofuran (or dimethylformamide) in the microwave oven. Under these conditions the formates as well as the acetate
Rapid Acid Hydrolysis of Albumin in a Microwave Oven
โ Scribed by A. Moralesrubio; J. Cerezo; A. Salvador; M. Delaguardia
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 325 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0026-265X
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โฆ Synopsis
Microwave-oven-assisted acid hydrolysis of albumin in (7.5 \mathrm{M} \mathrm{HCl}) was carried out in only (12 \mathrm{~min}) in a pressurized Teflon reactor using a power of (390 \mathrm{~W}). The effect of experimental conditions on the hydrolysis of albumin were evaluated and the results obtained were compared with those found by conventional hydrolysis, using in both cases the ninhydrin reaction for the spectrophotometric determination of free amino acids. A chromatographic study showed that microwave oven heating did not change the nature of the amino acids obtained from the hydrolysis of the protein. O 1993 Academic Press, Inc.
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