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rap1 p21 regulates the interaction of ras p21 with RGL, a new effector protein of ras p21

✍ Scribed by Masahiro Ikeda; Shinya Koyama; Michiko Okazaki; Kiyohiko Dohi; Akira Kikuchi

Book ID
115931502
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
447 KB
Volume
375
Category
Article
ISSN
0014-5793

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A method is described for the convenient preparation of substantial quantities of nucleotide-free p21 or of 1:l complexes with nucleotides other than GDP. The nucleotide-free protein has been used for kinetic studies of the binding of G D P and GTP, making use of the fluorescent analogues 3'-(methyl