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Kinetics of interaction of nucleotides with nucleotide-free H-ras p21

✍ Scribed by John, Jacob; Sohmen, Roland; Feuerstein, Juergen; Linke, Rosita; Wittinghofer, Alfred; Goody, Roger S.


Book ID
121348466
Publisher
American Chemical Society
Year
1990
Tongue
English
Weight
1014 KB
Volume
29
Category
Article
ISSN
0006-2960

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Kinetics of interaction of nucleotides w
✍ John, Jacob; Sohmen, Roland; Feuerstein, Juergen; Linke, Rosita; Wittinghofer, A πŸ“‚ Article πŸ“… 1990 πŸ› American Chemical Society 🌐 English βš– 1014 KB

A method is described for the convenient preparation of substantial quantities of nucleotide-free p21 or of 1:l complexes with nucleotides other than GDP. The nucleotide-free protein has been used for kinetic studies of the binding of G D P and GTP, making use of the fluorescent analogues 3'-(methyl