Kinetics of interaction of nucleotides w
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John, Jacob; Sohmen, Roland; Feuerstein, Juergen; Linke, Rosita; Wittinghofer, A
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Article
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1990
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American Chemical Society
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English
β 1014 KB
A method is described for the convenient preparation of substantial quantities of nucleotide-free p21 or of 1:l complexes with nucleotides other than GDP. The nucleotide-free protein has been used for kinetic studies of the binding of G D P and GTP, making use of the fluorescent analogues 3'-(methyl