Random coil chemical shift for intrinsically disordered proteins: effects of temperature and pH
✍ Scribed by Magnus Kjaergaard; Søren Brander; Flemming M. Poulsen
- Publisher
- Springer Netherlands
- Year
- 2011
- Tongue
- English
- Weight
- 547 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0925-2738
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## Abstract Accurate random coil α‐proton chemical shift values are essential for precise protein structure analysis using chemical shift index (CSI) calculations. The current study determines the chemical shift effects of pH, urea, peptide length and neighboring amino acids on the α‐proton of Ala
## Abstract __NMR spectroscopy of the full‐length neuronal Tau protein has proved to be difficult due to the length of the protein and the unfavorable amino acid composition. We show that the random‐coil chemical shift values and their dependence on the presence of a proline residue in the (__i__+1