Proline-Directed Random-Coil Chemical Shift Values as a Tool for the NMR Assignment of the Tau Phosphorylation Sites

✍ Scribed by Guy Lippens; Jean-Michel Wieruszeski; Arnaud Leroy; Caroline Smet; Alain Sillen; Luc Buée; Isabelle Landrieu

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 230 KB
• Volume: 5
• Category: Article
• ISSN: 1439-4227
• DOI: 10.1002/cbic.200300763

✦ Synopsis

Abstract

NMR spectroscopy of the full‐length neuronal Tau protein has proved to be difficult due to the length of the protein and the unfavorable amino acid composition. We show that the random‐coil chemical shift values and their dependence on the presence of a proline residue in the (i+1) position can successfully be exploited to assign all proline‐directed phosphorylation sites. This is a first step toward the study of the phosphorylation of Tau by NMR spectroscopy.