Radioiodination of sulfhydryl-sensitive proteins
โ Scribed by Colleen E. Hayes; Irwin J. Goldstein
- Book ID
- 107711693
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 310 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We present a simple method for determining the stoichiometry of three different proteins which had been radioiodinated using two different labeling agents. The method consists of developing a silica gel thin-layer chromatography plate containing an aliquot of the crude reaction mixture in ethanol an
A protocol is described for coupling of carrier-free iodine to protein sulfhydryl groups via fluorescein maleimide. 125I is first coupled to fluorescein maleimide in the presence of chloramine T. Iodination is stopped with sodium thiosulfate, and the iodine-substituted fluorescein maleimide is react