## Abstract A method is described for the tritium labelling of angiotensin II in two different positions. /Tyr‐^3^H/^4^‐angiotensin II and /Phe‐^3^H/^8^‐angiotensin II of high specific radioactivity have been prepared starting from Dbt^4^‐angiotensin II and Cpa^8^‐angiotensin II, respectively.
Radiation stability of angiotensin II-amide and of its tritiated derivatives
✍ Scribed by J. Kopoldová; Š. Hrnčíř; I. Mezö
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- French
- Weight
- 376 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
The gamma‐radiolysis of aqueous solutions of the peptide hormone angiotensin II‐amide and the beta‐autoradiolysis of its tritiated preparations were studied. The radiation loss and the decrease of biological activity of angiotensin II‐amide was evaluated. The main fractions of radiation products were separated and compared with the products formed during the storage of tritiated angiotensin II‐amide. The principal radiation processes taking place during the irradiation of angiotensin II‐amide were discussed.
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