The radial diffusion assay was evaluated for its usefulness as a simple, inexpensive assay for screening endopeptidase activity in vegetative plant tissues. Various substrates embedded in agar were tested with trypsin and a-chymotrypsin to select those with the greatest sensitivity for detecting pro
Radial diffusion method for determining tannin in plant extracts
โ Scribed by Ann E. Hagerman
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 962 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0098-0331
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๐ SIMILAR VOLUMES
The amount of arabinogalactan-protein in whole plant extracts can be quantified by single radial diffusion in agarose gels containing a dye known as the fl-glucosyl-Yariv reagent which specifically interacts with and precipitates arabinogalactan-proteins. The lower limit of quantification is 0.04 pg
A method to precipitate condensed tannin from crude plant extracts using trivalent ytterbium has been developed. The new method requires less time and resources than the condensed isolation procedure using Sephadex LH-20 recommended by Hagerman (1991, T annin Analysis, Miami University, Oxford, OH,
A method is described for manometric determination of bicarbonate (as little as 0.25 pmoles) in leaves. Conditions such as optimum pH and inactivation of carbonic anhydrase are introduced to stabilize the bicarbonate. Crude leaf extracts are purified with chloroform and Darco G-60. An ionic mixture