Quantification of arabinogalactan-protein in plant extracts by single radial gel diffusion

✍ Scribed by Gerrit-Jan van Holst; Adrienne E. Clarke

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 396 KB
• Volume: 148
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90251-9

✦ Synopsis

The amount of arabinogalactan-protein in whole plant extracts can be quantified by single radial diffusion in agarose gels containing a dye known as the fl-glucosyl-Yariv reagent which specifically interacts with and precipitates arabinogalactan-proteins. The lower limit of quantification is 0.04 pg of arabinogalactan-protein; gum arabic is used as a standard reference arabinogalactan-protein. In principle, this method can be adapted to measure levels of any dyeprecipitating macromolecule; for example, acidic polysaccharides can be estimated by their binding to the cationic dye Alcian blue. o 1985 Academic PRSS, I~C.