Manometric method for determining bicarbonate content in plant leaves

✍ Scribed by Chong W. Chang

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 455 KB
• Volume: 66
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90736-8

✦ Synopsis

A method is described for manometric determination of bicarbonate (as little as 0.25 pmoles) in leaves. Conditions such as optimum pH and inactivation of carbonic anhydrase are introduced to stabilize the bicarbonate. Crude leaf extracts are purified with chloroform and Darco G-60. An ionic mixture is separated from macromolecular compounds by elution from a column of Sephadex G-25. Carbon dioxide is released by acidifying the ionic mixture and is determined with a respirometer.

MATERIALS AND METHODS

Reagents.

Phosphate buffer, 5 mM at pH 8.0 containing 20.8 pg of sodium azide per ml (0.32 mM).

Chloroform.