Quinoline-induced chromosome aberrations and sister chromatid exchanges in rat liver

✍ Scribed by Shoji Asakura; Shigeki Sawada; Tadakazu Sugihara; Hirohiko Daimon; Fumio Sagami

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 80 KB
• Volume: 30
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)30:4<459::aid-em11>3.0.co;2-c

✦ Synopsis

Induction of chromosome aberrations and sister Treatment with 28 repeated doses of Q induced chromatid exchanges (SCEs) was studied in hepato-significant chromosome aberrations and SCEs dosecytes of F344 rats exposed in vivo to the hepatocar-dependently. Cytogenetic damage induced in the cinogen quinoline (Q). Hepatocytes were isolated liver by repeated doses of Q was greater than in-4-48 hr after a single dose of 200 mg/kg body duced by a single dose. Furthermore, Q induced weight or 24 hr after 28 repeated doses (once a replicative DNA synthesis in the liver, but failed to day) of 25-200 mg/kg body weight/day by gas-induce micronucleus formation in the bone marrow. tric intubation, and allowed to proliferate in Wil-The noncarcinogen 8-hydroxyquinoline was also liams' medium E supplemented with epidermal examined and found to be essentially non-genogrowth factor. Cells were fixed after a culture period toxic to rat liver. These results show that Q is a of 48 hr. A single dose of Q induced chromosome genotoxic carcinogen to rat liver and the present aberrations in up to 22% of metaphase cells, and method of in vivo cytogenetic assay should be useful SCEs with a frequency of up to 1.27 per chromo-for evaluating the genotoxicity of hepatocarcinosome 12 hr after the dose, while the control values gens.