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Quantitative ultramicroanalysis of amino acids in the form of their DNS-derivatives: II. On the use of the dansylation reaction for quantitative estimation of amino acids

โœ Scribed by V.A. Spivak; V.V. Shcherbukhin; V.M. Orlov; Ja.M. Varshavsky

Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
553 KB
Volume
39
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES

Quantitative ultramicroanalysis of amino
Quantitative ultramicroanalysis of amino acids in the form of their DNS-derivatives: I. An apparatus for ultramicroanalysis of DNS-amino acids
โœ V.A. Spivak; V.M. Orlov; V.V. Shcherbukhin; J.A.M. Varshavsky ๐Ÿ“‚ Article ๐Ÿ“… 1970 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 485 KB

The commonly used methods for amino acid analysis of peptides and proteins, including the method of Moore and Stein, require about 10v7 to 5 x 1O-g mole of sample. However, such amounts are not always available and there is an urgent demand for new and more sensitive methods of amino acid analysis.

Studies on the dansylation reaction by t
Studies on the dansylation reaction by the use of 14C-dansyl chloride: Application to analysis of free amino acids in the rat optic nerve
โœ Luigi Casola; G. Di Matteo ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 969 KB

Conditions are reported for labeling amino acids with "Cdansyl chloride. The technique has been used for the quantitative estimation of some amino acids present in amounts of optic nerve tissue as small as 0.1 mg. The results are very similar to those obtained with the amino acid autoanalyzer. Opti