𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Quantitative thin-layer chromatography : IX. Investigations on the reproducibility of direct spectrophotometry and fluorometry

✍ Scribed by H. Zürcher; G. Pataki; J. Borko; R.W. Frei

Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
467 KB
Volume
43
Category
Article
ISSN
1873-3778

No coin nor oath required. For personal study only.

✦ Synopsis

The reproducibility of direct scanning of thin-layer chromatograms has been investigated.

Provided the technique is rigidly standardized, relative standard deviations of I-5.3 % (on the same chromatogram) and 4-6 y0 (on different chromatograms) can be obtained in the case of reflectance spectroscopy, while fluorescence quenching measurements show relative standard deviation of 4-6 y. (on the same chromatogram) and 8.6-12 % (on different chromatograms). On different two-dimensional chromatograms relative standard deviations 6-12.5 y. (reflectance spectroscopy) and 13.5-15.2 y. (fluorescence quenching) have been observed. The reproducibility of fluorescence measurements has been found with two different instruments to be 1.9-3.6 y. and 2-5 %, respectively, on the same chromatogram, and 7.5 Ok and 9.1 O/J, respectively, on different chromatograms.

📜 SIMILAR VOLUMES

A new method for the determination of sa
A new method for the determination of saccharin based on thin-layer chromatography and spectrophotometry
✍ Vidaud, Z. E. ;García Roché, M. O. ;Gonzáles, Esther 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 201 KB 👁 1 views

Saccharin is extracted from the acidified liquid food with chloroform. This solvent is evaporated ; the residue is dissolved in ethyl acetate and applied on a silica gel GF,,, plate. After chromatographic separation, the saccharin band is scraped off and extracted with 1 % sodium carbonate solution.

Simple method for the quantitative analy
Simple method for the quantitative analysis of tetracyclines by direct fluorimetry after thin-layer chromatography on cellulose plates
✍ Enrico Ragazzi; Giovanni Veronese 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 641 KB

A thin-layer chromatographic method is described in which tetracyclines are separated on a cellulose layer by development with aqueous solutions of certain salts (magnesium, calcium, barium and zinc chloride). The spots exhibit good fluorescence in UV light, allowing ready detection without the nece

Quantitative thin-layer chromatography o
Quantitative thin-layer chromatography on liquid anion exchangers : Part I. An investigation into some of the parameters involved in the direct densitometric determination of zinc
✍ R.J.T. Graham; L.S. Bark; D.A. Tinsley 📂 Article 📅 1969 🏛 Elsevier Science 🌐 English ⚖ 695 KB

A study has been made of some of the parameters involved in the direct densitometric determination of zinc on cellulose layers impregnated with the liquid ion exchanger Primene JM-T hydrochloride. The chromogenic reagent PAN was employed to locate the zinc spots. Of the factors associated with the