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Simple method for the quantitative analysis of tetracyclines by direct fluorimetry after thin-layer chromatography on cellulose plates

โœ Scribed by Enrico Ragazzi; Giovanni Veronese


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
641 KB
Volume
132
Category
Article
ISSN
1873-3778

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โœฆ Synopsis


A thin-layer chromatographic method is described in which tetracyclines are separated on a cellulose layer by development with aqueous solutions of certain salts (magnesium, calcium, barium and zinc chloride). The spots exhibit good fluorescence in UV light, allowing ready detection without the necessity for treatment with any reagents. This fluorescence can be used for the direct photometric determination of the tetracyclines with good accuracy.


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