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Simple method for the analysis of food dyes on reversed-phase thin-layer plates

✍ Scribed by Hisao Oka; Yoshitomo Ikai; Norihisa Kawamura; Masuo Yamada; Hiromasa Inoue; Tsutomu Ohno; Kensuke Inagaki; Arifumi Kuno; Nagisa Yamamoto

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 953 KB
Volume: 411
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)93996-7

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✦ Synopsis

A technique for the determination of food dyes using reversed-phase thin-layer chromatography on octadecyl-modified silica is described. A solvent system containing 5.0% aqueous sodium sulphate solution enables good separation of the food dyes. Their separation is dependent on the pH of the solvent system, good separation among all dyes being obtained between pH 6.0 and 7.0. The determination of thirteen dyes can be achieved by a combination of methanol-acetonitrile-5.0% aqueous sodium sulphate solution (3:3:10) (solvent system A) and methanol-methyl ethyl ketone-5.0% aqueous sodium sulphate solution (1: 1: 1) (solvent system B).

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