Quantitative thin-layer chromatography on liquid anion exchangers : Part I. An investigation into some of the parameters involved in the direct densitometric determination of zinc
✍ Scribed by R.J.T. Graham; L.S. Bark; D.A. Tinsley
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 695 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A study has been made of some of the parameters involved in the direct densitometric determination of zinc on cellulose layers impregnated with the liquid ion exchanger Primene JM-T hydrochloride.
The chromogenic reagent PAN was employed to locate the zinc spots.
Of the factors associated with the instrument (the Joyce-Loebl Chromoscan) the most important has been shown to be the setting bf the baseline co&rol. The most important factor concerned with the chromatographic system is shown to be the difficulty of distributing the chromogenic reagent uniformly over the layer.
In spite of these problems it has been shown that an accuracy of about 5 y0 can be achieved at the I pg level.
', ,In, scanning the standard spots a 10 mm x I mm slit aperture was .used thrdughout. Unless otherwise stated each standard spot was scanned twenty-five times and the integrator counts quoted represent the mean counts.