𝔖 Bobbio Scriptorium
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Quantitative separation of starch components on a cellulose column

✍ Scribed by N.B. Patil; N.R. Kale

Book ID
104145954
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
719 KB
Volume
84
Category
Article
ISSN
1873-3778

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✦ Synopsis

Ethanol-induced adsorption of amylose on a cellulose column equilibrated with ethanol-urea is used for the quantitative separation of amylose and amylopectin from starch. Under these conditions, amylose is completely retained on the column. The column is washed free of amylopectin and the adsorbed amylose is eluted by gradient elution with ethanol-urea.

Samples containing 300-400 mg of starch can be successfully fractionated on a column packed with defatted cellulose (20 g) with a recovery of 95k2o/o. The elution profile reveals the heterogeneity of native amylose.

πŸ“œ SIMILAR VOLUMES

Separation of the components of starch b
Separation of the components of starch based on the adsorption of the 1-butanolβ€”amylose complex during column chromatography on cellulose
✍ Nima D. Kamath; Shobhana V. Bhide; Narayan R. Kale πŸ“‚ Article πŸ“… 1989 πŸ› Elsevier Science 🌐 English βš– 537 KB

Amylose and amylopectin from defatted, dispersed starch can be separated quantitatively by column chromatography on cellulose, using 0.1~ acetate buffer (pH 4.8) containing 8.5% of 1-butanol and 2~ urea at 30". The amylose-1-butanol complex is adsorbed and the amylopectin-1-butanol complex is eluted