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Separation of the components of starch based on the adsorption of the 1-butanol—amylose complex during column chromatography on cellulose

✍ Scribed by Nima D. Kamath; Shobhana V. Bhide; Narayan R. Kale

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 537 KB
Volume: 186
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(89)84011-x

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✦ Synopsis

Amylose and amylopectin from defatted, dispersed starch can be separated quantitatively by column chromatography on cellulose, using 0.1~ acetate buffer (pH 4.8) containing 8.5% of 1-butanol and 2~ urea at 30". The amylose-1-butanol complex is adsorbed and the amylopectin-1-butanol complex is eluted. The amylose is then eluted by decreasing the concentration of 1-butanol in the eluent. The asymmetric elution profile of amylose indicates heterogeneity as judged by the blue values, beta-amylolysis limits, and the action of pullulanase on the various fractions.

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