Analytical and semipreparative high performance liquid chromatography separation of stereoisomers of novel 3,4-dihydropyrimidin-4(3H)-one derivatives on the immobilised amylose-based Chiralpak IA chiral stationary phase
✍ Scribed by Roberto Cirilli; Rosella Ferretti; Bruno Gallinella; Francesco La Torre; Antonello Mai; Dante Rotili
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 982 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
Direct HPLC separation of stereoisomers of three novel 5‐methyl‐2‐(alkylthio)‐6‐(2,6‐difluorophenylalkyl)‐3,4‐dihydropyrimidin‐4(3__H__)‐ones endowed with antiviral and potential antiproliferative and morphological differentiation activity against melanoma cells was performed by using the new immobilised amylose‐based Chiralpak IA chiral stationary phase. Stereoselective conditions were achieved using normal phase eluents containing “non‐standard” solvents such as ethyl acetate, methyl tert‐butyl ether, or dichloromethane. In order to study the chiroptical properties of single stereoisomers, mg‐scale separations were performed on analytical and semipreparative size Chiralpak IA columns in combination with ethyl acetate‐based eluents.