Quantitative profiling of the metabolic cascade of arachidonic acid by capillary gas chromatography mass spectrometry

✍ Scribed by J. Roselló; E. Gelpi; M. Rigaud; J. Durand; J. C. Breton

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 458 KB
• Volume: 8
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200080404

✦ Synopsis

The analytical approach described allows for the rapid screening and concurrent quantitative determination of all of the major metabolites of the cyclooxygenase pathway of arachidonic acid, including the primary prostaglandins PGE,, PGD2 and PGF,, in addition to the stable end-products of short-lived prostacyclin and thromboxane A, (6-keto-PGF1, and TXB2, respectively), generated by incubating arachidonic acid with mouse peritoneal macrophage cells. After derivatization into the corresponding methyl ester-methoxime-TMS derivative, the extracted endogenous compounds were analysed by combined high efficiency' glass capillary column chromatography and selected ion monitoring. Comparatively lower detection limits can be achieved with the methyl ester-butylboronate-TMS derivatives (e.g. 10 pg for 6-keto-PGF1,). A brief account is also presented on a new type of mixed methyl ester-pentafluorobenzyloxime-butylboronate-TMS derivative of TXBz and 6-keto-PGF1,.