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Quantitative PCR determination of human cytomegalovirus in blood cells

✍ Scribed by J. Satou; T. Funato; N. Satoh; Y. Abe; K.K. Ishii; T. Sasaki; M. Kaku

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
71 KB
Volume
15
Category
Article
ISSN
0887-8013

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✦ Synopsis

Abstract

We evaluated a rapid and sensitive method to determine human cytomegalovirus (CMV) DNA levels in blood cells using a quantitative polymerase chain reaction (PCR) technique. This method is based on real‐time detection of PCR using a dual fluorescence‐labeled probe and a sequence detector. Ten copies of CMV DNA were detected, when 1 μg of DNA from blood samples was used with this method, and a good correlation was obtained between increased concentrations of copy numbers calculated and measured copy numbers of CMV DNA (r = 0.999). Forty normal subjects exhibited no copies of CMV DNA. On the other hand, a 6‐month‐old girl tested positive for increased levels 4 weeks after liver transplant. This method is simple, accurate, and sensitive for the quantitative detection of CMV DNA in vivo, indicating possible applications for the diagnosis and monitoring of CMV infection. J. Clin. Lab. Anal. 15:122–126, 2001. © 2001 Wiley‐Liss, Inc.

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