Quantitative determination of coenzyme Q10 in human blood for clinical studies

✍ Scribed by Surasi Vadhanavikit; Naoki Sakamoto; Nobuyuki Ashida; Takeo Kishi; Karl Folkers

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 311 KB
• Volume: 142
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90530-x

✦ Synopsis

A quantitative method for the determination of coenzyme QIo (CoQ3 in human blood has been devised which allows recovery of essentially 10% of the CoQ,,. The use of whole blood rather than plasma includes the CoQr,, in white cells. The method utilizes TLC instead of saponification to fractionate lipid impurities, because CoQra is sensitive to saponification, and utilizes CoQ, , as an internal standard which is advantageous over Cot&, and a synthetic quinone. The final step of HPLC frequently reveals a peak with a retention time like that of CoQs which, being less than that of CoQIo, can be near other peaks of impurities. o 1984 Academic PWS, I~C.