## Most investigators favor, for isolation of total glyeogen from animal tissues, the modification of Good et al. (1) of Pfluger's method (2), and assay the precipitate with the anthrone reagent (3). This combination of procedures yielded low and unreproducible results when applied to 0.1 mg of gl
Quantitative measurement of starch in very small amounts of leaf tissue
β Scribed by J. C. MacRae
- Publisher
- Springer-Verlag
- Year
- 1971
- Tongue
- English
- Weight
- 392 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
A specific enzyme method is described for the routine estimation of starch in small quantities (10-30 mg) of dried leaf tissue. A fl-glucanase-free preparation of amyloghicosidase is employed to hydrolyse starch to glucose; this is subsequently estimated by the glucose oxidase technique. The method gives result which agree closely with those obtained by a specific iodine-precipitation method.
π SIMILAR VOLUMES
The enzyme nitrite reductase (EC 1.6.6.4) is generally assayed in terms of disappearance of nitrite from the assay medium. We describe a technique which allowed estimation of the enzyme level in leaf tissues of Vigna mungo (L). Hepper in terms of the release of the product (NH3) of the enzyme reacti
## Abstract Six methods for estimating starch were applied to three samples of lucerne leaflet tissue. Three of the methods, each incorporating the specific glucoseβoxidase procedure to estimate the starch hydrolysis products, gave values in close agreement with each other. These values were lower