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Measurement of nitrite reductase in leaf tissue ofVigna mungo

✍ Scribed by R. C. Srivastava; Bandana Bose; D. Mukerji; S. N. Mathur; H. S. Srivastava

Publisher: Springer-Verlag
Year: 1979
Tongue: English
Weight: 226 KB
Volume: 147
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00388737

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✦ Synopsis

The enzyme nitrite reductase (EC 1.6.6.4) is generally assayed in terms of disappearance of nitrite from the assay medium. We describe a technique which allowed estimation of the enzyme level in leaf tissues of Vigna mungo (L). Hepper in terms of the release of the product (NH3) of the enzyme reaction. The technique is offered as an alternative, possibly more convenient method for assay of nitrite reductase in plant tissue in vivo.

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